Quantitative molecular and culture analyses of bacterial elimination in oval-shaped root canals by a single-file instrumentation technique

Authors: F. R. F. Alves, I. N. Rocas, M. A. S. Neves, J. Zoffoli, B. M. Almeida, J. F. Siqueira
Year: 2012
Journal:
Rubrics: Bacterial reduction
Keywords: oval shaped root canals

Aim Bacterial reduction in oval-shaped root canals by a single-instrument technique was compared ex vivo with a conventional nickel–titanium rotary technique. Data obtained from two quantification methods, quantitative real-time polymerase chain reaction (qPCR) and culture, were also compared.

Methodology Oval-shaped canals of extracted teeth contaminated with Enterococcus faecalis were instrumented using either a single Reciproc instrument or the BioRaCe instrument series. Bacteriological samples were taken before (S1) and after instrumentation (S2). Bacterial quantification was performed using qPCR and culture.

Results Intragroup analysis showed that both protocols promoted a highly significant bacterial reduction (P < 0.001). Intergroup analysis (S2 samples) showed no significant differences between the two instrumentation systems (P > 0.05). As for the quantification methods, qPCR revealed significantly higher counts of E. faecalis in S1 than culture (P < 0.05), but no significant differences occurred for S2 (P > 0.05).

Conclusion The single-file technique was comparable with the conventional technique in oval-shaped canals provided the width of apical preparation, volume of irrigants and duration of irrigation are kept similar. No significant difference was observed for qPCR and culture in post-instrumentation samples, indicating that both methods can be reliably used for studies of antibacterial effectiveness. Keywords: endodontic treatment, oval root canals, single-file instrumentation, sodium hypochlorite.. 10

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