Authors: A. C. S. Marinho, F. C. Martinho, L. M. Gonçalves, H. R. C. Rabang, B. P. F. A. Gomes
Rubrics: Cleaning effectiveness
Keywords: bacterial reduction, disinfection, endodontic treatment, endotoxin, reciprocating motion, rotary instruments
Introduction: To evaluate the effectiveness of Reciproc for the removal of cultivable bacteria and endotoxins from root canals in comparison with multifile rotary systems.
Methods: The root canals of forty human single-rooted mandibular pre-molars were contaminated with an Escherichia coli suspension for 21 days and randomly assigned to four groups according to the instrumentation system: GI – Reciproc (VDW); GII – Mtwo (VDW); GIII – ProTaper Universal (Dentsply Maillefer); and GIV –FKG Race™ (FKG Dentaire) (n = 10 per group). Bacterial and endotoxin samples were taken with a sterile/apyrogenic paper point before (s1) and after instrumentation (s2). Culture techniques determined the colony-forming units (CFU) and the Limulus Amebocyte Lysate assay was used for endotoxin quantification. Results were submitted to paired t-test and anova.
Results: At s1, bacteria and endotoxins were recovered in 100% of the root canals investigated (40/40). After instrumentation, all systems were associated with a highly significant reduction of the bacterial load and endotoxin levels, respectively: GI – Reciproc (99.34% and 91.69%); GII – Mtwo (99.86% and 83.11%); GIII – ProTaper (99.93% and 78.56%) and GIV – FKG Race™ (99.99% and 82.52%) (P < 0.001). No statistical difference were found amongst the instrumentation systems regarding bacteria and endotoxin removal (P > 0.01).
Conclusion: The reciprocating single file, Reciproc, was as effective as the multifile rotary systems for the removal of bacteria and endotoxins from root canals.